【預期用途】 本產品用于定量測定人(ren)血(xue)清或血(xue)漿（肝素鋰抗凝）中的(de)人(ren)體促黃體生成激(ji)素。

該產(chan)品(pin)是(shi)一(yi)種(zhong)在(zai)VIDAS系(xi)列(lie)(lie)分析儀上使用(yong)(yong)的自動定(ding)量試(shi)驗，使用(yong)(yong)ELFA技術（酶(mei)聯熒光分析），定(ding)量檢測(ce)(ce)人(ren)血(xue)清或(huo)血(xue)漿（肝素(su)(su)鋰作(zuo)為(wei)抗凝(ning)劑(ji)）中的人(ren)體(ti)(ti)(ti)(ti)促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)。 促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)（LH）是(shi)一(yi)種(zhong)糖(tang)蛋白(bai)，分子(zi)量大(da)約30,000道爾頓(dun)，它有(you)(you)兩個多(duo)肽亞(ya)(ya)單(dan)位（α和(he)β），通過(guo)非共價鍵連接在(zai)一(yi)起形成(cheng)(cheng)(cheng)促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)。促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)的α亞(ya)(ya)單(dan)位由(you)(you)92個氨基酸(suan)組成(cheng)(cheng)(cheng)，與促(cu)(cu)(cu)卵泡激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)（FSH）、甲狀腺(xian)(xian)(xian)(xian)刺(ci)(ci)激(ji)(ji)(ji)(ji)(ji)(ji)(ji)激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)（TSH）以(yi)(yi)及人(ren)絨毛膜促(cu)(cu)(cu)性(xing)(xing)(xing)腺(xian)(xian)(xian)(xian)激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)(hCG)的α亞(ya)(ya)單(dan)序列(lie)(lie)相同(tong)。β亞(ya)(ya)單(dan)位由(you)(you)121個氨基酸(suan)組成(cheng)(cheng)(cheng)，包(bao)含*的氨基酸(suan)序列(lie)(lie)，它使促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)有(you)(you)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物特異性(xing)(xing)(xing)和(he)免(mian)疫特異性(xing)(xing)(xing)。促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)分子(zi)還包(bao)括(kuo)碳水(shui)化合(he)物，占該激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)分子(zi)量20％。1，2 促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)由(you)(you)垂體(ti)(ti)(ti)(ti)前葉(xie)促(cu)(cu)(cu)性(xing)(xing)(xing)腺(xian)(xian)(xian)(xian)細胞(bao)以(yi)(yi)脈沖方(fang)式分泌并(bing)由(you)(you)下丘腦控制(zhi)。促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)刺(ci)(ci)激(ji)(ji)(ji)(ji)(ji)(ji)(ji)產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)類(lei)固醇激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)（男性(xing)(xing)(xing)為(wei)睪酮，女性(xing)(xing)(xing)為(wei)雌(ci)二(er)醇），而類(lei)固醇激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)對促(cu)(cu)(cu)性(xing)(xing)(xing)腺(xian)(xian)(xian)(xian)激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)有(you)(you)負反饋作(zuo)用(yong)(yong)。在(zai)男性(xing)(xing)(xing)，促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)通過(guo)刺(ci)(ci)激(ji)(ji)(ji)(ji)(ji)(ji)(ji)Leydig細胞(bao)產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)睪酮。在(zai)經期婦女，促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)與促(cu)(cu)(cu)卵泡激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)協同(tong)作(zuo)用(yong)(yong)直(zhi)接影響卵泡的發(fa)育，增加(jia)它們(men)的類(lei)固醇產(chan)物并(bing)且決定(ding)排(pai)卵。在(zai)絕經期, 雌(ci)二(er)醇的生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)劇烈下降，導致促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)血(xue)清水(shui)平的升(sheng)(sheng)高(gao)。3，4 無論是(shi)否結合(he)促(cu)(cu)(cu)卵泡激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)，促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)測(ce)(ce)定(ding)法都是(shi)一(yi)個研(yan)究生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)殖功(gong)能(neng)的重要(yao)參數。對于(yu)男性(xing)(xing)(xing)和(he)閉經的婦女升(sheng)(sheng)高(gao)的促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)水(shui)平說(shuo)明主要(yao)的性(xing)(xing)(xing)腺(xian)(xian)(xian)(xian)功(gong)能(neng)減(jian)(jian)退(tui)，較低水(shui)平的促(cu)(cu)(cu)黃(huang)(huang)體(ti)(ti)(ti)(ti)(生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng))激(ji)(ji)(ji)(ji)(ji)(ji)(ji)素(su)(su)說(shuo)明次要(yao)的性(xing)(xing)(xing)腺(xian)(xian)(xian)(xian)功(gong)能(neng)減(jian)(jian)退(tui)（低促(cu)(cu)(cu)性(xing)(xing)(xing)腺(xian)(xian)(xian)(xian)素(su)(su)性(xing)(xing)(xing)功(gong)能(neng)減(jian)(jian)退(tui)癥(zheng)）。5,6,7

【檢驗原(yuan)理】 采用(yong)將(jiang)酶(mei)免疫夾心分析方(fang)法與(yu)最后熒(ying)光(guang)檢測(ELFA)相結(jie)合的分析原(yuan)理。

固相管（SPR®）在分析中除作為反應的固相表面，也(ye)作為移液(ye)裝(zhuang)置(zhi)。反應試(shi)劑(ji)為即用型(xing)并事先加入到試(shi)劑(ji)條的孔中，再(zai)將(jiang)孔密封。 所有分析步(bu)驟都由儀器自動進行。反應物進出SPR數次(ci)。

首先，將(jiang)樣本加入到含有堿性磷酸酶(mei)標(biao)記的(de)抗(kang)促黃(huang)體(生成(cheng))激素(LH)抗(kang)體（共(gong)扼物）的(de)孔中(zhong)。樣品/共(gong)扼物混合(he)(he)物循環進出SPR數(shu)次，以提高(gao)反應速度。抗(kang)原結合(he)(he)到包被(bei)在(zai)SPR的(de)抗(kang)體上(shang)，并結合(he)(he)到共(gong)扼物上(shang)，以形成(cheng)“夾心"。 沒有結合(he)(he)的(de)組分(fen)在(zai)沖洗(xi)步(bu)驟(zou)中(zhong)消(xiao)除。

在最后(hou)檢測(ce)步驟當中，底物(wu)（磷酸4-甲(jia)基傘(san)形(xing)烷(wan)）循環進出(chu)SPR。共扼(e)物(wu)酶催化(hua)水解底物(wu)成(cheng)熒(ying)光產(chan)物(wu)（4-甲(jia)基傘(san)形(xing)酮），在450nm處測(ce)量其熒(ying)光強度。熒(ying)光強度與出(chu)現在標本(ben)中抗原的濃度成(cheng)正(zheng)比。 檢測(ce)結束后(hou)的時候(hou)，根據儲存(cun)在存(cun)儲器(qi)中的校正(zheng)曲線，儀器(qi)動(dong)計算結果，然后(hou)打(da)印輸出(chu)。

梅里埃30407 促卵泡激素檢測試劑盒

梅里埃30407 促卵泡激素檢測試劑盒